MED 3LAB BIOCHEM & MEDICAL BIOLOGY

Worksheet 4: Attempt review My LMS Subjects / 2021-PSB-MED3LAB(SP-T3-PT) / Assessment / Worksheet 4 Started on Thursday, 28 October 2021, 12:05 AM State Finished Completed on Friday, 29 October 2021, 5:41 PM Time taken 1 day 17 hours Grade 19.50 out of 21.50 (91%) Information Laboratory Module 4 Assessment In this laboratory module, you prepared plasmid DNA (i.e. performed a “miniprep”) from overnight cultures of positive GFP-producing and negative non-GFP-producing transformed ce...

Worksheet 3: Attempt review My LMS Subjects / 2021-PSB-MED3LAB(SP-T3-PT) / Assessment / Worksheet 3 Started on Tuesday, 12 October 2021, 10:45 PM State Finished Completed on Wednesday, 13 October 2021, 11:27 PM Time taken 1 day Marks 19.50/19.50 Grade 24.50 out of 24.50 (100%) Information Laboratory Module 3 Assessment In this laboratory module, you prepared growth media and agar plates. You also prepared competent E. coli cells for plasmid transformation using the heat shock method....

Worksheet 2: Attempt review My LMS Subjects / 2021-PSB-MED3LAB(SP-T2-FT) / Assessment / Worksheet 2 Started on Saturday, 12 June 2021, 12:55 AM State Finished Completed on Tuesday, 15 June 2021, 10:59 AM Time taken 3 days 10 hours Grade 24.75 out of 25.50 (97%) Information Worksheet - Constructing the pQE30-GFP expression vector In this exercise, you relate both the theoretical and practical aspects of the protocols used for restriction enzyme digestion, DNA purification, gel electrop...

Worksheet 1: Attempt review My LMS Subjects / 2021-PSB-MED3LAB(SP-T2-FT) / Assessment / Worksheet 1 Started on Friday, 4 June 2021, 6:00 PM State Finished Completed on Sunday, 6 June 2021, 5:33 PM Time taken 1 day 23 hours Grade 12.00 out of 15.00 (80%) Information Worksheet 1 One of your first tasks in the practicals is to perform a PCR to obtain large quantities of the GFP cDNA from the pE-GFP vector. Following digestion with appropriate restriction enzymes, this DNA will be ligate...

Topic 1 Introduction to Molecular Cloning 1. The goal of this integrated practical course is to purify a protein. In general, what is the value of protein purification to medical research? What information can a purified protein provide and what techniques can be developed/employed once you have a purified protein?  In vitro studies - Small molecule interactions, protein binding partners etc  Producing antibodies  Determination of structure Purification of the protein is a valua...

PURIFICATION OF HEXAHISTIDINE TAGGED GFP PROTEIN FROM TRANSFORMED E.COLI XL1 CELLS USING IMMOBILIZED METAL AFFINITY CHROMATOGRAPHY Lee Jia Yi (12) Abstract In this study, a recombinant histidine-tagged green fluorescence protein(His6-GFP) was produced by E.coli XL1 cells followed by immobilized metal affinity chromatography (IMAC) using Nickel-agarose beads for purification. The GFP template was first amplified from pE-GFP vector using polymerase chain reaction (PCR). The primers c...

PURIFICATOIN BY IMAC OF A SUB-CLONED AND EXPRESSED GFP Aranza Lopez Group B Friday The green fluorescent protein (GFP) of the jellyfish Aequorea Victoria is commonly used as a reporter molecule for localization and expression (Tsien, 1998). To gain experience in current techniques in molecular and medical cell biology, the open reading frame (ORF) of a GFP was sub-cloned into a bacterial expression vector and then purified by immobilized metal affinity chromatography (IMAC). At the e...