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Exam (elaborations)
ASCP BOC EXAM Study Guide
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---11June 20232022/2023A+
- ASCP BOC EXAM Study GuideWhen using an electronic cell counter, which of the following results can occur in the presence of a cold agglutination? a) increased MCV and decreased RBC b) increased MCV and normal RBC c) decreased MCV and increased MCHC d) decreased MCV and RBC - ANSWERa) increased MCV and decreased RBC 
 
Cerebrospinal fluid for glucose assay should be: a) refrigerated b) analyzed immediately c) heated to 56 degree C d) stored at room temp after centrifugation - ANSWERb) analyzed im...
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AMINAHASSAN001
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Exam (elaborations)
MB ASCP Ch 13 Molecular Detection of Inherited Diseases Estimatics Exam.
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---5June 20232022/2023
- MB ASCP Ch 13 Molecular Detection of Inherited Diseases Estimatics En polymorphisms -ANSWER mutations useful for mapping, determining parentage 
 
balanced polymorphisms -ANSWER just a DNA sequence difference, the phenotypic effect of which is counteracted by a second trait or polymorphism 
 
methylation -ANSWER usually cytosine in CpG islands, shuts down RNA transcription by adding methyl groups 
 
genomic impringing -ANSWER enzymatic addition of methyl groups to specific nitrogen bases in a pr...
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AMINAHASSAN001
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Exam (elaborations)
Practice test questions for MBASCP updated version.
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---29June 20232022/2023A+
- Practice test questions for MBASCP updated version.Mutation in this gene (Xp21) is associated with Duchenne Muscular Dystrophy: 
a. Dystrophin 
b. P53 
c. Musculin 
d. C-myc - a. Dystrophin 
 
This restriction enzyme "digests and adds a methyl group from Adenine": 
Type I 
Type II 
Type III 
DNase I - Type III 
 
What links codons and anti-codons together during DNA translation? 
 
 DNA ligase 
 Single-stranded binding protein 
 Complementary base pairing 
 GTP - Complementary base pairing 
 
...
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AMINAHASSAN001
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Exam (elaborations)
Molecular Diagnostics Chapter 4(Well Solved).
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---4June 20232022/2023A+
- Molecular Diagnostics Chapter 4(Well Solved). 
gel electrophoresis -ANSWERS. Procedure used to separate and analyze DNA fragments by placing a mixture of DNA fragments at one end of a porous gel and applying an electrical voltage to the gel 
 
Is the anode positive or negative? What about the cathode? -ANSWERS. Positive, negative 
 
DNA/RNA are positively/negatively charged? They migrate towards anode or cathode? -ANSWERS. negatively, anode 
 
Do large or small size DNA fragments migrate faster?...
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AMINAHASSAN001
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Exam (elaborations)
Molecular Diagnostics Chapter 11 ORIGINAL VERSION.
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---5June 20232022/2023A+
- Molecular Diagnostics Chapter 11 ORIGINAL VERSION. 
What are examples of PCR inhibitors? - ANSWERS.hemoglobin, acidic polysacharrides, nitrate, crystals, beta-human chorionic gonadatropin, heparin, lactoferrin, anticoagulants, sodium polyanethol sulfonate, and polyamines 
 
STAR - ANSWERS.stool transport and recovery buffer inactivates infectious organisms 
 
What is the purpose of a sensitivity control? - ANSWERS.shows lower limit of detection 
 
true negative - ANSWERS.Indicates accurately tha...
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AMINAHASSAN001
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Exam (elaborations)
Molecular Diagnostics Chapter 7(All Correct Answers Avaible).
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---5June 20232022/2023A+
- Molecular Diagnostics Chapter 7(All Correct Answers Avaible)chromosome mutation - ANSWERS.change in chromosome structure 
 
Genome mutations - ANSWERS.changes in chromosome number 
 
Euploid - ANSWERS.an individual with How many base pairs are in the human genome? - ANSWERS.2.9 billion 
 
What is the difference between a mutation and a polymorphism? - ANSWERS.mutations are reserved for rare changes in DNA like those found in cancer. Polymorphisms are variants that occur in 1-2% of the population...
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AMINAHASSAN001
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Exam (elaborations)
Translocations Advanced 2023.
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---1June 20232022/2023A+
- Translocations Advanced 2023.t(1,13) - ANSWERPAX3/7 + FKHR (there are two of these) 
Rhabdomyosarcoma 
 
t(2:13) - ANSWERPAX3/7 + FKHR (there are two of these) 
Rhabdomyosarcoma 
 
t(11:22) - ANSWEREwing sarcoma, childhood neoplasms, EWSR1 
 
t(x:18) - ANSWERsynovial sarcoma, SS18, reciprocal translocation 
ss18/syt—>ssx1/ssx2 
 
t(9:22) - ANSWERPhiladelphia chromosome, CML (BCR-ABL activation, tyrosine kinase oncogene) 
 
t(15:17) - ANSWERacute promyelocytic leukemia 
PML/RARA 
 
t(8:14) ...
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AMINAHASSAN001
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Exam (elaborations)
Molecular Diagnostics Chapter 5(revised edition).
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---7June 20232022/2023A+
- Molecular Diagnostics Chapter 5(revised edition).What type of restriction enzyme is commonly used in the laboratory? - ANSWERtype II, 4-6 bp recognition sites 
 
restriction map - ANSWERdiagram that shows the lengths of fragments between restriction sites in the strand of DNA 
 
If you cut a linear fragment of DNA with a restriction enzyme at three sites, how many fragments do you have? What about if you cut a plasmid at three sites - ANSWER4 fragments, 3 fragments 
 
How do you perform restrict...
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AMINAHASSAN001
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Exam (elaborations)
Certification Exam Cards(complete solution graded).
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---43June 20232022/2023A+
- Certification Exam Cards(complete solution graded).When handling blood spots, one should 
 A. be even more careful when handling blood than other body fluids. 
 B. protect from cross contamination from other blood spots 
 C. store at room temperature up to 24 hours 
 D. store at 4 degrees C for up to 7 years - ANSWERB. protect from cross contamination from other blood spots 
 
In handling blood serum, one should 
 
A. store in eppendorf tube in refrigerator indefinitely 
B. separate from ce...
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AMINAHASSAN001
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Exam (elaborations)
Molecular Diagnostics Chapter 13 Assessment.
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---6June 20232022/2023A+
- Molecular Diagnostics Chapter 13 Assessment.Oncogenes 
genes that cause cancer by blocking the normal controls on cell reproduction, oncogenes prevent apoptosis
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AMINAHASSAN001