BIOL340

BIOL340 CELL DEATH PATHWAYS EXAM QUESTIONS AND ANSWERS WITH COMPLETE SOLUTIONS GRADED A++ LATEST UPDATE cell death introduction critical for normal physiology - Embryogenesis - Tissue homeostasis Critical for removing damaged or diseased cells (development of cancer) Unwarranted cell death as a result of disease = Neurodegeneration Impaired cell death (Cancer or Autoimmune disease) Neurodegeneration The loss of particular neurons via apoptosis. can also prevent cell death 3 ma...

BIOL340 - REPLICATION #1 EXAM QUESTIONS AND ANSWERS WITH COMPLETE SOLUTIONS VERIFIED LATEST UPDATE What are the three activities associated with DNA replication? Initiation, Elongation, Termination What is Initiation? Recognition of an origin, separation and stabilization of parental strands. What is Elongation? Assembly of the replisome at the replication fork, synthesis of the daughter strands. What is Termination? Joining at the ends, separation of the chromosome. What is the origi...

BIOL340 - QUIZ 3 EXAM QUESTIONS AND ANSWERS WITH COMPLETE SOLUTIONS VERIFIED LATEST UPDATE what are the 2 phases in the cell cycle, and which one takes the longest 1. Interphase: G1, S, G2 2. M phase - interphase is the longest What happens in G1 phase cell growth and organelles duplicate What happens in S phase DNA and chromosomes replicate What happens in G2 phase cell grows, preparing for mitosis and centrosome duplicates and chromosomes condense what happens in M phase = mito...

BIOL340 QUIZ 1 EXAM QUESTIONS AND ANSWERS WITH COMPLETE SOLUTIONS VERIFIED what is light microscopy - how does it work visible light passes through specimen, glass lenses refract the light to magnify the specimen what is the resolution limit of light microscopy around 300 nm name the different types of light microscopy Brightfield (unstained), Brightfield (stained), Fluorescence, Phase contrast, Differential interference contrast, Confocal what is brightfield; unstained vs stained unst...

BIOL340 SPECIAL EXAM QUESTIONS AND ANSWERS WITH COMPLETE SOLUTIONS GRADED A++ Proton positively charged particle; inside the nucleus; considerable weight compared to other components of an atom Neutron neutrally charged particle; inside the nucleus; considerable weight compared to other components of an atom Electron negatively charged particle; outside the nucleus; weighs significantly less than other particles Bohr Model model of atomic structure; Electrons move around the nucleus in...

BIOL340 DAZZA EXAM QUESTIONS AND ANSWERS WITH COMPLETE SOLUTIONS GRADED A++ LATEST UPDATE meselson-stahl experiment grew bacteria in 15N, washed and put in 14N, 2nd gen 1/2 way band, 3rd gen just 14N (not disp as 1/2 way band, not cons as new band) prokaryote dna replication topisomerase cuts and loosens dna, initiator proteins bind to OriC, helicase binds and unwinds using atp, primase binds, dna pol 3 synthesises, pol 1 removes primer using nick translation and ligase seals okazaki fragm...

BIOL340 CELL CYCLE EXAM QUESTIONS AND ANSWERS WITH COMPLETE SOLUTIONS GRADED A++ LATEST UPDATE Basic premise of signal transduction Trying to get a message from outside the cell to generate a response inside the cell What does CDK stand for cyclin dependent kinase what is the basic premise of cyclins To cycle proteins during the cell cycle. what is the basic premise of CDKs Proteins that depend on cyclins Universal requirement of somatic cells must divide, producing one copy of their...

QUIZ 1 BIOL340 EXAM QUESTIONS AND ANSWERS WITH COMPLETE SOLUTIONS GRADED A++ LATEST UPDATE Cell membranes Definition Any membrane found in a cell, including the plasma membrane (PM) and many other membranes surrounding organelles and cytoplasmic structures, which creates compartments Membrane functions (7) - Compartmentalization (closing off small areas of cell to be specialised for functions) - Scaffold for biochemical activities - Selectively permeable barrier (NOT to water) - Transpo...

BIOL340 - PETER EXAM QUESTIONS AND ANSWERS WITH COMPLETE SOLUTIONS GRADED A++ LATEST UPDATE Why sequence a genome - Understand the function and evolution of an organism (evolution of genome and evolution of relationship with different species) - Find out how genes work together to direct growth, development, and maintenance of an organism - Find correlations between how genome information relates to development of cancer, susceptibility to certain diseases and drug metabolism (pharmacogeno...

BIOL340 EXAM QUESTIONS AND ANSWERS WITH COMPLETE SOLUTIONS GRADED A++ real time pcr/qpcr amplifies and measures dna in real time using fluorescent markers, only allows relative quantification to house keeping gene methods of measuring mrna abundance in extracts by rnaseq if you dont have prior genetic info, microarrays if you know the target gene of interest, qrt-pcr (ct and ddpcr), in situ hybridisation for exact location reverse transcription pcr rt rna into cdna to be pcred what quan...