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Title: GE09 Unknown Pathogen Analysis Objective: To identify an unknown pathogen(s) based on the given assays developed during this course Procedure: 1. See recorded results of various tests 2. identify the two pathogens in question Notes: Two samples from the hospital were brought to your lab for diagnosis. Another coworker began the analysis after culturing the samples on LB agar plates. However, your coworker left you the remaining work due to an emergency. Before leaving, your coworke...

Title: GE07 Secondary Assays for Bacterial Characterization Objective: To profile bacterial population based on additional biochemical approaches to better determine growth/metabolic characteristics Procedure: Indole Test: Test #1 1. pick an isolated colony from BAP 2. using a sterile loop. Smear directly onto the reaction area of the slide 3. Examine the test area for colour change within 20 seconds Indole Test: Test #2 1. Using a sterile loop, pick an isolated colony from BAP

Title: GE06 Enzymatic Assays Objective: To profile bacterial populations based on key enzymatic reactions and determine growth/metabolic characteristics Procedure: -Oxidase test 1. Using a sterile loop, pick an isolated colony from BAP 2. Smear directly onto the reaction area of the slide 3. Examine the test area for colour change within 20 seconds -Catalase test 1. Using a sterile loop, carefully pick the colony from the plate 2. Smear colony directly onto the glass slide 3. Add two d...

BIOD 171 LAB 5 NOTE Title: GE05 Testing bacteria for antibiotic sensitivity Objective: to determine the threshold of antibiotic sensitivity across bacterial strains using the Kirby-Bauer method (aka standardized disc susceptibility test) Procedure: 1. Streak bacteria A across an LB agar plate for confluent growth using a sterile L spreader 2. Evenly place the paper antibiotic discs on the plate 3. Invert the plate and incubate at 37C overnight Notes: Materials needed: non-selectiv...

BIOD 171 LAB 4 NOTE Title: GE04 Growth Media Objective: To understand the types and uses of growth media for isolating and identifying unknown bacterial samples. Procedure: 4-phase dilution streaking: clonal isolation 1. Using sterile loop spread culture in area #1 2. Using the new sterile loop, drag through the end of area #1 once 3. Using the new sterile loop, drag through the end of area #2 once 4. Using a new sterile loop, drag through the end of area #3 once. Use a back-and-forth p...

BIOD 171 LAB 3 NOTE Title: GE03 Mounting Techniques Objective: Microscopic explanation of bacterial samples through various staining techniques. Identify the colour and shape of given samples. Procedure: For a dry mount: 1. Clean slide (70%ethanol) 2. Circle area on the slide for easy location of the specimen (optional) 3. Apply the organism to the slide: If it is from culture, use a sterile loop to spread it onto the slide. If it is from the plate, use a sterile loop to pick colonies and...

BIOD 171 LAB 2 NOTE Objective: to become familiar with the basic components if a light microscope as well as how to load a sample for viewing Procedure: 1. Review parts & components 2. Load sample slide onto microscope 3. Select magnification 4. Make necessary adjustments to optimize sample visualization Notes: Magnify glass, lens, light source, object to look at Basic parts of microscope: eyepiece, arm/neck, objective, revolving nosepiece, stage, stage controls, stage clips, course/fine ...

Which of the following are considered Protista? Select all that apply. Amoeba Algae A defining characteristic of Protista is the inability of colonies to form tissue layers. True ATP In phosphorylation, the light reactions always occur where? Membrane The Calvin cycle must occur in the absence of light. False The function of the ribosome is (select all that apply): Protein Synthesis Identify the following cellular components by matching the number with the descrip...

1. If disease occurrence in a population is described as disperse with rare occurrence in a population, it is considered: Sporadic 2. Cystic fibrosis, which runs in families, is an example of a disease with what type of etiology? Genetic 3. According to Koch's postulates, what is the significance of causing disease in a healthy subject with a pure culture of the pathogen? It satisfies the need for experimental evidence linking the pathogen to the

Name the two main divisions of the digestive system organs. 2. Name the seven parts of the gastrointestinal tract. 3. Name the six digestive system accessory organs. 4. Review all the figures and diagrams, including the GI tract and accessory organs. 1 (a) Alimentary canal, (b) the accessory digestive organs 2. Mouth, Pharynx, stomach, small intestine, large intestine, anus, rectum 3. Teeth, tongue, gall bladder, pancreas, liver,salivary gland 4 .done